| 1. | The plant genetic engineering and its application in agriculture
植物基因工程及其在农业中的应用 |
| 2. | National laboratory of protein engineering and plant genetic engineering , peking university , beijing , 100871
北京大学蛋白质及植物基因工程国家重点实验室，北京， 100871 |
| 3. | The national laboratory of protein engineering and plant genetic engineering , college of life sciences , peking university , beijing , 100871
北京大学生命科学学院，蛋白质工程及植物基因工程国家重点实验室，北京， 100871 |
| 4. | Through comparison detailed information about the characteristics of the two promoters can also be obtained , which is necessary for precisely regulated gene expression in plant genetic engineering
通过对种子特异性启动子的比较可加深对其表达模式的了解，为植物基因工程中的精细调控提供依据。 2 |
| 5. | The seeds of plants , especially the oil plants and corn , are related very closely to human life and , therefore , are becoming important target material for plant genetic engineering
摘要植物的种子，尤其是油料和谷类作物的种子与人类的生产生活关系非常密切，因此，也成为植物基因工程中进行改良的重要目标材料。 |
| 6. | The study of the structure and function of these promoters will help not only to comprehend the development of seeds and the mechanisms of gene expression and organic substance accumulation but also search for some promoter elements useful for plant genetic engineering
对这类启动子的结构和功能元件的了解，有助于人们更加深入地理解高等植物基因表达调控机制，提高人们对植物种子发育过程及有机物在种子中积累机制的认识，而且可以为植物基因工程中生物反应器的研究提供有应用价值的启动子元件。 |
| 7. | Abstract : the low expression level of foreign genes in transgenic plants is a puzzling question for researchers in plant genetic engineering . the biosafety concern is now causing a growing public wariness of transgenic plants around the world . this article reviews the new strategies currently used in construction of plant expression vector for plant genetic engineering . these strategies are important for obtaining better expression of foreign genes and developing safer transgenic plants
摘要外源基因在转基因植物中表达效率低一直是令相关研究者困扰的一个问题.转基因植物的生物安全性最近在全球范围内开始引起世人的担忧.本文简要介绍了近年来在植物表达载体构建方面所采用的一些新策略,这些策略有助于增强外源基因的表达水平、提高生物工程体的安全性。 |
| 8. | With its broad - spectrum and non - specific potent antibacterial activity , cationic antibacterial peptide has the potential to be widely used in plant disease resistance genetic engineering . however , cema was refrained in plant genetic engineering application due to its toxic to plant cells . the modification of cema will facilitate its wide application
由于其抗菌谱广、杀菌力强等优点，在植物抗病基因工程中有广阔的应用前景，但由于对植物细胞有毒害作用， cema在植物遗传转化中的应用受到很大的限制。 |
| 9. | By means of plant genetic engineering , foreign insects resistance gene can be transferred into plant cell . we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method . and obtained the transgenic mustard plants . the main results are as follows : 1 . isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves . the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers
本实验是利用植物基因工程获得抗虫的转基因芥菜植株，结果如下： 1豇豆胰蛋白酶抑制剂基因的分离分别提取豇豆种子、子叶及叶片的总rna ，逆转录成cdna 。以豇豆胰蛋白酶抑制剂基因两端的序列为引物，用rt - pcr的热启动方法从上述cdna中扩增出目的基因片断。 |
